Effect of Selected Antioxidants on the In Vitro Aging of Human Fibroblasts.
Int J Mol Sci
; 25(3)2024 Jan 26.
Article
em En
| MEDLINE
| ID: mdl-38338809
ABSTRACT
The modification of the replicative lifespan (RLS) of fibroblasts is of interest both from a knowledge point of view and for the attenuation of skin aging. The effect of six antioxidants at a concentration of 1 µM on the replicative lifespan of human dermal fibroblasts was studied. The nitroxide 4-hydroxy-TEMPO (TEMPOL), ergothioneine, and Trolox extended the replicative lifespan (RLS) (40 ± 1 population doublings (PD)) by 7 ± 2, 4 ± 1, and 3 ± 1 PD and lowered the expression of p21 at late passages. Coumaric acid, curcumin and resveratrol did not affect the RLS . The level of reactive oxygen species (ROS) was decreased or not affected by the antioxidants although TEMPOL and coumaric acid decreased the level of glutathione. Only ergothioneine and resveratrol decreased the level of protein carbonylation. The antioxidants that could prolong the RLS elevated the mitochondrial membrane potential. Protecting the activity of mitochondria seems to be important for maintaining the replicative capacity of fibroblasts.
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Base de dados:
MEDLINE
Assunto principal:
Marcadores de Spin
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Óxidos N-Cíclicos
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Ergotioneína
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Antioxidantes
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article