Generation of induced pluripotent stem cell line (VRISGi004-A) from a healthy female donor by reprogramming erythroid progenitor cells.
Stem Cell Res
; 76: 103331, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38341988
ABSTRACT
We generated a human induced pluripotent stem cell (hiPSC) line from erythroid progenitor cells (EPCs) of a 20-year-old female healthy donor using Sendai virus vector encoding Yamanaka factors OCT3/4, SOX2, c-MYC, and KLF4. The established hiPSCs showed a standard morphology and expression of typical undifferentiated stem cell markers, a normal karyotype (46, XX), and demonstrated potential for differentiation in vitro. Furthermore, they were successfully differentiated into cardiomyocytes that expressed cardiomyocyte-specific markers. The iPSC line and iPSC-derived cardiomyocytes will provide new avenues for future drug testing/development and personalized cell therapy for cardiovascular diseases (CVDs).
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Base de dados:
MEDLINE
Assunto principal:
Doenças Cardiovasculares
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Células-Tronco Pluripotentes Induzidas
Limite:
Adult
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Female
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Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article