Methods to extract and analyze fluid from human pluripotent stem cell-derived choroid plexus organoids.
Front Mol Neurosci
; 16: 1243499, 2023.
Article
em En
| MEDLINE
| ID: mdl-38348236
ABSTRACT
The choroid plexus (ChP) is a highly vascularized tissue lining the ventricular space of the brain. The ChP generates cerebrospinal fluid (CSF) and forms a protective barrier in the central nervous system (CNS). Recently, a three-dimensional human pluripotent stem cell (hPSC)-derived ChP organoid model has been developed. This model generates cystic structures that are filled with a fluid resembling CSF and are surrounded by an epithelial layer expressing ependymal choroid plexus-specific markers. Here we describe a method to generate these choroid plexus organoids using a commercial kit and methods to extract the CSF-like fluid for use in downstream analysis.
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MEDLINE
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En
Ano de publicação:
2023
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Article