An efficient ELISA protocol for measurement of SARS-CoV-2 spike-specific IgG in human plasma and serum samples.
MethodsX
; 12: 102596, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38379717
ABSTRACT
Here, we describe a protocol for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike-specific immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA). The protocol was developed with a keen focus on optimizing several key parameters, including antigen coating concentration, antibody and sample dilutions, and assay development time. The final protocol features the following characteristicsâ¢The capability to detect SARS-CoV-2 spike-specific IgG in both plasma and serum samples.â¢A streamlined procedure that requires only 1 hour and 20 minutes of hands-on time.â¢Reliable assay performance, with a remarkable sensitivity of 98.1 % and specificity of 99.5 %.
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2024
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Article