Synchronous force and Ca2+ measurements for repeated characterization of excitation-contraction coupling in human myocardium.
Commun Biol
; 7(1): 220, 2024 Feb 22.
Article
em En
| MEDLINE
| ID: mdl-38388802
ABSTRACT
Dysfunctional Ca2+ signaling affects the myocardial systole and diastole, may trigger arrhythmia and cause transcriptomic and proteomic modifications in heart failure. Thus, synchronous real-time measurement of Ca2+ and force is essential to investigate the relationship between contractility and Ca2+ signaling and the alteration of excitation-contraction coupling (ECC) in human failing myocardium. Here, we present a method for synchronized acquisition of intracellular Ca2+ and contraction force in long-term cultivated slices of human failing myocardium. Synchronous time series of contraction force and intracellular Ca2+ were used to calculate force-calcium loops and to analyze the dynamic alterations of ECC in response to various pacing frequencies, post-pause potentiation, high mechanical preload and pharmacological interventions in human failing myocardium. We provide an approach to simultaneously and repeatedly investigate alterations of contractility and Ca2+ signals in long-term cultured myocardium, which will allow detecting the effects of electrophysiological or pharmacological interventions on human myocardial ECC.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteômica
/
Insuficiência Cardíaca
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article