Targeting deoxynivalenol for degradation by a chimeric manganese peroxidase/glutathione system.
Ecotoxicol Environ Saf
; 273: 116130, 2024 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-38394761
ABSTRACT
The manganese peroxidase (MnP) can degrade multiple mycotoxins including deoxynivalenol (DON) efficiently; however, the lignin components abundant in foods and feeds were discovered to interfere with DON catalysis. Herein, using MnP from Ceriporiopsis subvermispora (CsMnP) as a model, it was demonstrated that desired catalysis of DON, but not futile reactions with lignin, in the reaction systems containing feeds could be achieved by engineering MnP and supplementing with a boosting reactant. Specifically, two successive strategies (including the fusion of CsMnP to a DON-recognizing ScFv and identification of glutathione as a specific targeting enhancer) were combined to overcome the lignin competition, which together resulted into elevation of the degradation rate from 2.5% to as high as 82.7% in the feeds. The method to construct a targeting MnP and fortify it with an additional enhancer could be similarly applied to catalyze the many other mycotoxins with yet unknown responsive biocatalysts.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tricotecenos
/
Lignina
/
Micotoxinas
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article