Performance assessment of a new G12/A1 antibody-based rapid ELISA using commercially available and gluten-spiked food samples.
Eur J Gastroenterol Hepatol
; 36(4): 416-422, 2024 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-38407849
ABSTRACT
OBJECTIVE:
Food products with <20â mg/kg gluten can be labeled 'gluten-free' according to international regulations. Several antibodies-based ELISAs have been develop to track gluten traces in food products. Among them, R5 and G12 antibody-based ELISAs are the frequently used methods. However, these antibodies have certain limitations. We evaluated the accuracy of G12/A1 antibody-based 'Glutentox ELISA Rapid G12' and compared the results with the current reference method i.e., R5 antibody-based 'Ridascreen R5 ELISA'.METHODS:
In the first step, the performance of Glutentox ELISA Rapid G12 kit was inspected by determination of the threshold value i.e., > or <20â mg/kg gluten in different food products. In the second step, quantification accuracy was assessed by quantification of gluten in gluten-free food products spiked with gliadin reference material.RESULTS:
In total 47 food products (naturally and labeled gluten-free, and food with traces of gluten) were included. Of them, 29 products were quantified with <20â mg/kg, and 18 with a low level of gluten by both the kits. Six out of 29 gluten-free products were used for the recovery test at different spike levels. Gluten concentration and mean recovery rates of individual kits showed consistency.CONCLUSION:
GlutenTox Rapid G12 ELISA could be an appropriate choice for detecting gluten in food products but needs more in-house validation and collaborative tests.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Análise de Alimentos
/
Glutens
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article