Reduction-to-synthesis: the dominant approach to genome-scale synthetic biology.
Trends Biotechnol
; 42(8): 1048-1063, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-38423803
ABSTRACT
Advances in systems and synthetic biology have propelled the construction of reduced bacterial genomes. Genome reduction was initially focused on exploring properties of minimal genomes, but more recently it has been deployed as an engineering strategy to enhance strain performance. This review provides the latest updates on reduced genomes, focusing on dual-track approaches of top-down reduction and bottom-up synthesis for their construction. Using cases from studies that are based on established industrial workhorse strains, we discuss the construction of a series of synthetic phenotypes that are candidates for biotechnological applications. Finally, we address the possible uses of reduced genomes for biotechnological applications and the needed future research directions that may ultimately lead to the total synthesis of rationally designed genomes.
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Base de dados:
MEDLINE
Assunto principal:
Genoma Bacteriano
/
Biologia Sintética
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article