Buffalo colostrum peptide mitigates Parkinson's disease pathophysiology through Cullin-3 inhibition.
Bioorg Chem
; 145: 107242, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38428285
ABSTRACT
Colostrum/Milk is a chief repertoire of antioxidant peptides. Nuclear factor-erythroid 2 related factor 2 (Nrf2) is a viable target for Parkinson's Disease (PD), as this pathway deduced to be impaired in PD. Cullin-3 is one of the crucial E3 ligase responsible for its regulation. The present study screened peptide libraries of buffalo colostrum & milk peptides for Cullin-3 inhibition, thus ensuing activation of Nrf2 to alleviate the molecular etiopathology in PD using the C. elegans as a model. The structure was modelled, binding sites analyzed and peptide-interactions analyzed by docking. Among the 55 sequences (≤1 kDa), the peptide SFVSEVPEL having the highest dock score (-16.919) was synthesized and evaluated for its effects on oxidative stress markers, antioxidant enzymes, neurochemical marker and Nrf2/Skn-1 levels. The lead peptide alleviated the oxidative pathophysiology and behavioural deficits associated with PD in C. elegans.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doença de Parkinson
/
Fármacos Neuroprotetores
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article