High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm.
J Reprod Dev
; 70(2): 131-137, 2024 Apr 04.
Article
em En
| MEDLINE
| ID: mdl-38432992
ABSTRACT
Cold transport of the cauda epididymides is a useful technique for shipping laboratory rat sperm. Cold transport of rat sperm avoids potential risks of microbiological infection, animal escape or death, and animal welfare issues. Previously, we reported that a cold-storage solution containing dimethyl sulfoxide and quercetin maintained the fertility of cold-stored rat sperm. However, cold-stored rat sperm exhibited a decreased fertilization rate after 24-h storage. To recover the fertility of cold-stored sperm, we focused on the effects of bovine serum albumin (BSA), a cholesterol acceptor that induces sperm capacitation. We sought to determine the optimal concentration of BSA in fertilization medium based on the fertility of cold-stored rat sperm. High concentrations of BSA (40 mg/ml) enhanced the fertilization rate of cold-stored rat sperm and maintained sperm fertility for 144 h. Embryos derived from cold-stored and BSA-treated sperm normally developed into pups after embryo transfer. In summary, high BSA concentrations enhanced the fertility of cold-stored rat sperm and prolonged the storage period to 144 h, thereby expanding the transportable region for genetically engineered rats.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Soroalbumina Bovina
/
Fertilização in vitro
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article