Near-Infrared Perylenecarboximide Fluorophores for Live-Cell Super-Resolution Imaging.
J Am Chem Soc
; 146(11): 7135-7139, 2024 03 20.
Article
em En
| MEDLINE
| ID: mdl-38441879
ABSTRACT
Organic near-infrared (NIR) photoblinking fluorophores are highly desirable for live-cell super-resolution imaging based on single-molecule localization microscopy (SMLM). Herein we introduce a novel small chromophore, PMIP, through the fusion of perylenecarboximide with 2,2-dimetheylpyrimidine. PMIP exhibits an emission maximum at 732 nm with a high fluorescence quantum yield of 60% in the wavelength range of 700-1000 nm and excellent photoblinking without any additives. With resorcinol-functionalized PMIP (PMIP-OH), NIR SMLM imaging of lysosomes is demonstrated for the first time in living mammalian cells under physiological conditions. Moreover, metabolically labeled nascent DNA is site-specifically detected using azido-functionalized PMIP (PMIP-N3) via click chemistry, thereby enabling the super-resolution imaging of nascent DNA in phosphate-buffered saline with a 9-fold improvement in spatial resolution. These results indicate the potential of PMIP-based NIR blinking fluorophores for biological applications of SMLM.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Corantes Fluorescentes
/
Imagem Individual de Molécula
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article