New Zealand blackcurrant extract modulates the heat shock response in men during exercise in hot ambient conditions.
Eur J Appl Physiol
; 124(8): 2315-2328, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-38448730
ABSTRACT
PURPOSE:
To determine if 7d of New Zealand blackcurrant (NZBC) extract alters the heat shock, inflammatory and apoptotic response during prolonged exertional-heat stress.METHODS:
Ten men (Age 29 ± 2 years, Stature 1.82 ± 0.02 m, Mass 80.3 ± 2.7 kg, VÌO2max 56 ± 2 mL·kg-1·min-1) ingested two capsules of CurraNZ™ (NZBC extract 210 mg anthocyanins·day-1) or PLACEBO for 7d prior to 1 h treadmill run (65% VÌO2max) in hot ambient conditions (34 °C/40% RH). Blood samples were collected before (Pre), immediately after (Post), 1 h after (1-Post), and 4 h after (4-Post) exercise. Heat shock proteins (HSP90, HSP70, HSP32) were measured in plasma. HSP and protein markers of inflammatory capacity (TLR4, NF-κB) and apoptosis (BAX/BCL-2, Caspase 9) were measured in peripheral blood mononuclear cells (PBMC).RESULTS:
eHSP32 was elevated at baseline in NZBC(+ 31%; p < 0.001). In PLACEBO HSP32 content in PBMC was elevated at 4-Post(+ 98%; p = 0.002), whereas in NZBC it fell at Post(- 45%; p = 0.030) and 1-Post(- 48%; p = 0.026). eHSP70 was increased at Post in PLACEBO(+ 55.6%, p = 0.001) and NZBC (+ 50.7%, p = 0.010). eHSP90 was increased at Post(+ 77.9%, p < 0.001) and 1-Post(+ 73.2%, p < 0.001) in PLACEBO, with similar increases being shown in NZBC (+ 49.0%, p = 0.006 and + 66.2%, p = 0.001; respectively). TLR4 and NF-κB were both elevated in NZBC at PRE(+ 54%, p = 0.003 and + 57%, p = 0.004; respectively). Main effects of study condition were also shown for BAX/BCL-2(p = 0.025) and Caspase 9 (p = 0.043); both were higher in NZBC.CONCLUSION:
7d of NZBC extract supplementation increased eHSP32 and PBMC HSP32 content. It also increased inflammatory and apoptotic markers in PBMC, suggesting that NZBC supports the putative inflammatory response that accompanies exertional-heat stress.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
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Exercício Físico
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Apoptose
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Resposta ao Choque Térmico
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Temperatura Alta
Limite:
Adult
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Humans
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Male
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article