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The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes.
Chen, Ru-Yi; Zhao, Teng; Guo, Jing-Jing; Zhu, Feng; Zhang, Na-Na; Li, Xiao-Feng; Liu, Hai-Tao; Wang, Fei; Deng, Yong-Qiang; Qin, Cheng-Feng.
Afiliação
  • Chen RY; College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, China; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China.
  • Zhao T; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China.
  • Guo JJ; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China.
  • Zhu F; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China; School of Life Sciences, Southwest Forestry University, Kunming, 650224, China.
  • Zhang NN; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China.
  • Li XF; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China.
  • Liu HT; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China.
  • Wang F; College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, 330045, China. Electronic address: wangfei179@163.com.
  • Deng YQ; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China. Electronic address: dengyq1977@126.com.
  • Qin CF; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China. Electronic address: qincf@bmi.ac.cn.
Virol Sin ; 39(2): 228-234, 2024 Apr.
Article em En | MEDLINE | ID: mdl-38461965
ABSTRACT
Guaico Culex virus (GCXV) is a newly identified segmented Jingmenvirus from Culex spp. mosquitoes in Central and South America. The genome of GCXV is composed of four or five single-stranded positive RNA segments. However, the infection kinetics and transmission capability of GCXV in mosquitoes remain unknown. In this study, we used reverse genetics to rescue two GCXVs (4S and 5S) that contained four and five RNA segments, respectively, in C6/36 â€‹cells. Further in vitro characterization revealed that the two GCXVs exhibited comparable replication kinetics, protein expression and viral titers. Importantly, GCXV RNAs were detected in the bodies, salivary glands, midguts and ovaries of Culex quinquefasciatus at 4-10 days after oral infection. In addition, two GCXVs can colonize Cx. quinquefasciatus eggs, resulting in positive rates of 15%-35% for the second gonotrophic cycle. In conclusion, our results demonstrated that GCXVs with four or five RNA segments can be detected in Cx. quinquefasciatus eggs during the first and second gonotrophic cycles after oral infection.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Replicação Viral / RNA Viral / Culex / Mosquitos Vetores Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Replicação Viral / RNA Viral / Culex / Mosquitos Vetores Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article