Protocol for xenografting of BxPC-3 pancreatic tumors using a chorioallantoic membrane model.
STAR Protoc
; 5(2): 102968, 2024 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-38492226
ABSTRACT
The chorioallantoic membrane (CAM) model is an increasingly attractive model for the study of human tumors. However, concise techniques for the use of pancreatic ductal adenocarcinoma BxPC-3 xenografts in CAM assays are not yet available. Here, we present a protocol for the induction of BxPC-3 xenograft tumors with high grafting efficiency. We describe steps for embryo incubation, egg handling, and grafting, each of which has been optimized to prevent fungal contamination and minimize mortality.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Membrana Corioalantoide
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article