Sumatriptan mitigates bleomycin-induced lung fibrosis in male rats: Involvement of inflammation, oxidative stress and α-SMA.
Tissue Cell
; 88: 102349, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38492426
ABSTRACT
INTRODUCTION:
Idiopathic pulmonary fibrosis (IPF) is a fibrotic lung condition that produces symptoms including coughing which may cause by excessive accumulation of scar tissue inflammatory and oxidative stress exacerbation. Sumatriptan, utilized for migraine treatment as a selective 5-HT1B/1D receptor agonist, has demonstrated significant anti-inflammatory and antioxidant properties in multiple preclinical investigations. Operating primarily on serotonin receptors, sumatriptan leverages the diverse physiological functions of serotonin, playing a pivotal role in regulating both inflammation and oxidative stress which is particularly relevant in the context of IPF. MATERIALS &METHODS:
Thirty-five male Wistar rats were divided to five group, including Sham (without IPF induction), control (BLM 5â¯mg/kg, intraperitoneally), and three fibrosis group with sumatriptan (0.5, 1, and 3â¯mg/kg, i.p. for 2 weeks) administration. IPF was induced by injection of BLM (single dose, 5â¯mg/kg intratracheally). Lung tissues were separated for measurement of myeloperoxidase (MPO) as an oxidative stress hallmark, and tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-ß), and transforming growth factor-ß (TGF-ß) as inflammatory markers as well as alpha smooth muscle actin (α-SMA). Also, for histological investigations, tissue damages were assessed by Hematoxylin-eosin (H&E) and Masson's trichrome staining method.RESULTS:
BLM-induced fibrosis could increase α-SMA, MPO, TNF-α, IL-1ß, and TGF-ß, while treatment with sumatriptan has reversed the α-SMA, MPO, and IL-1ß levels. Moreover, the results of H&E and Masson's trichrome staining indicated that sumatriptan (1 and 3â¯mg/kg) reduced tissue damages, alveolar wall thickness, collagen accumulation, and pulmonary fibrosis induced by BLM.CONCLUSION:
According to the data achieved from this study, Sumatriptan appears to have therapeutic benefits in IPF, possibly via reducing α-SMA as well as inflammation and the toxicity caused by oxidative stress.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Fibrose Pulmonar
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Bleomicina
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Actinas
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Ratos Wistar
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Sumatriptana
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Estresse Oxidativo
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Inflamação
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article