Scd1 and monounsaturated lipids are required for autophagy and survival of adipocytes.
Mol Metab
; 83: 101916, 2024 May.
Article
em En
| MEDLINE
| ID: mdl-38492843
ABSTRACT
OBJECTIVE:
Exposure of adipocytes to 'cool' temperatures often found in the periphery of the body induces expression of Stearoyl-CoA Desaturase-1 (Scd1), an enzyme that converts saturated fatty acids to monounsaturated fatty acids. The goal of this study is to further investigate the roles of Scd in adipocytes.METHOD:
In this study, we employed Scd1 knockout cells and mouse models, along with pharmacological Scd1 inhibition to dissect the enzyme's function in adipocyte physiology.RESULTS:
Our study reveals that production of monounsaturated lipids by Scd1 is necessary for fusion of autophagosomes to lysosomes and that with a Scd1-deficiency, autophagosomes accumulate. In addition, Scd1-deficiency impairs lysosomal and autolysosomal acidification resulting in vacuole accumulation and eventual cell death. Blocking autophagosome formation or supplementation with monounsaturated fatty acids maintains vitality of Scd1-deficient adipocytes.CONCLUSION:
This study demonstrates the indispensable role of Scd1 in adipocyte survival, with its inhibition in vivo triggering autophagy-dependent cell death and its depletion in vivo leading to the loss of bone marrow adipocytes.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Autofagia
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Estearoil-CoA Dessaturase
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Ácidos Graxos Monoinsaturados
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Camundongos Knockout
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Adipócitos
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article