Construction of recombinant fluorescent LSDV for high-throughput screening of antiviral drugs.
Vet Res
; 55(1): 33, 2024 Mar 16.
Article
em En
| MEDLINE
| ID: mdl-38493160
ABSTRACT
Lumpy skin disease virus (LSDV) infection is a major socio-economic issue that seriously threatens the global cattle-farming industry. Here, a recombinant virus LSDV-ΔTK/EGFP, expressing enhanced green fluorescent protein (EGFP), was constructed with a homologous recombination system and applied to the high-throughput screening of antiviral drugs. LSDV-ΔTK/EGFP replicates in various kidney cell lines, consistent with wild-type LSDV. The cytopathic effect, viral particle morphology, and growth performance of LSDV-ΔTK/EGFP are consistent with those of wild-type LSDV. High-throughput screening allowed to identify several molecules that inhibit LSDV-ΔTK/EGFP replication. The strong inhibitory effect of theaflavin on LSDV was identified when 100 antiviral drugs were screened in vitro. An infection time analysis showed that theaflavin plays a role in the entry of LSDV into cells and in subsequent viral replication stages. The development of this recombinant virus will contribute to the development of LSDV-directed antiviral drugs and the study of viral replication and mechanisms of action.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Bovinos
/
Doença Nodular Cutânea
/
Vírus da Doença Nodular Cutânea
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article