Temozolomide nano-in-nanofiber delivery system with sustained release and enhanced cellular uptake by U87MG cells.
Drug Dev Ind Pharm
; 50(5): 420-431, 2024 May.
Article
em En
| MEDLINE
| ID: mdl-38502031
ABSTRACT
OBJECTIVE:
The study was aimed at formulating temozolomide (TMZ) loaded gelatin nanoparticles (GNPs) encapsulated into polyvinyl alcohol (PVA) nanofibers (TMZ-GNPs-PVA NFs) as the nano-in-nanofiber delivery system. The secondary objective was to explore the sustained releasing ability of this system and to assess its enhanced cellular uptake against U87MG glioma cells in vitro.SIGNIFICANCE:
Nano-in-nanofibers are the emerging drug delivery systems for treating a wide range of diseases including cancers as they overcome the challenges experienced by nanoparticles and nanofibers alone.METHODS:
The drug-loaded GNPs were formulated by one-step desolvation method. The Design of Experiments (DoE) was used to optimize nanoparticle size and entrapment efficiency. The optimized drug-loaded nanoparticles were then encapsulated within nanofibers using blend electrospinning technique. The U87MG glioma cells were used to investigate the uptake of the formulation.RESULTS:
A 32 factorial design was used to optimize the mean particle size (145.7 nm) and entrapment efficiency (87.6%) of the TMZ-loaded GNPs which were subsequently ingrained into PVA nanofibers by electrospinning technique. The delivery system achieved a sustained drug release for up to seven days (in vitro). The SEM results ensured that the expected nano-in-nanofiber delivery system was achieved. The uptake of TMZ-GNPs-PVA NFs by cells was increased by a factor of 1.964 compared to that of the pure drug.CONCLUSION:
The nano-in-nanofiber drug delivery system is a potentially useful therapeutic strategy for the management of glioblastoma multiforme.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tamanho da Partícula
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Álcool de Polivinil
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Sistemas de Liberação de Medicamentos
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Preparações de Ação Retardada
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Nanopartículas
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Nanofibras
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Liberação Controlada de Fármacos
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Temozolomida
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article