PCR testing for herpesviruses in aqueous humor samples from patients with and without clinical corneal endothelial graft rejection.
J Med Virol
; 96(3): e29538, 2024 Mar.
Article
em En
| MEDLINE
| ID: mdl-38506230
ABSTRACT
To compare prevalence of positive PCR tests for herpesviruses between patients with and without a history of clinical corneal endothelial allograft rejection (AGR). Retrospective cross-sectional study with two-group comparison. A total of 307 aqueous humor (AH) samples from 235 Patients and 244 eyes who underwent penetrating keratoplasty or Descemet membrane endothelial keratoplasty or had a diagnostic AH aspiration due to clinical AGR between 2019 and 2023 were tested for DNA of herpes simplex virus (HSV), varicella-zoster virus (VZV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV). PCR test results were compared between the two groups (with/without AGR). Another sub-analysis examined the results of patients without a history of herpetic keratitis. A total of 8% of eyes with clinical AGR (9/108) had a positive PCR result for one of the herpesviruses (HSV3, CMV3, EBV2, VZV1). All patients in the group without AGR had negative PCR results for all previous viruses (0/136). The difference was statistically significant (p < 0.001). The sub-analysis of eyes without a history of herpetic keratitis also revealed significantly more positive herpes PCR results (7/87) in eyes with AGR than in eyes without AGR (0/42, p = 0.005). Clinical AGR after keratoplasty shows a significant correlation to viral replication. Herpetic infection and AGR could occur simultaneously and act synergistically. Timely differentiation between active herpetic infection and/or AGR is pivotal for proper treatment and graft preservation.
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Base de dados:
MEDLINE
Assunto principal:
Ceratite Herpética
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Infecções por Citomegalovirus
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Infecções por Herpesviridae
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Infecções por Vírus Epstein-Barr
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article