Efficient axonal transport of endolysosomes relies on the balanced ratio of microtubule tyrosination and detyrosination.
J Cell Sci
; 137(8)2024 04 15.
Article
em En
| MEDLINE
| ID: mdl-38525600
ABSTRACT
In neurons, the microtubule (MT) cytoskeleton forms the basis for long-distance protein transport from the cell body into and out of dendrites and axons. To maintain neuronal polarity, the axon initial segment (AIS) serves as a physical barrier, separating the axon from the somatodendritic compartment and acting as a filter for axonal cargo. Selective trafficking is further instructed by axonal enrichment of MT post-translational modifications, which affect MT dynamics and the activity of motor proteins. Here, we compared two knockout mouse lines lacking the respective enzymes for MT tyrosination and detyrosination, and found that both knockouts led to a shortening of the AIS. Neurons from both lines also showed an increased immobile fraction of endolysosomes present in the axon, whereas mobile organelles displayed shortened run distances in the retrograde direction. Overall, our results highlight the importance of maintaining the balance of tyrosinated and detyrosinated MTs for proper AIS length and axonal transport processes.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transporte Axonal
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Tirosina
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Camundongos Knockout
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Lisossomos
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Microtúbulos
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article