Effect of vitamin D3 supplementation on blood parameters and liver gene expression in female rats.
Mol Biol Rep
; 51(1): 456, 2024 Mar 27.
Article
em En
| MEDLINE
| ID: mdl-38536498
ABSTRACT
BACKGROUND:
To better understand the molecular mechanism responsible for the therapeutic potential of vitamin D, we conducted an analysis of the liver transcriptomes of adult female rats.METHODS:
Adult female rats (n = 18) were divided into three groups, receiving different doses of vitamin D group I, 0; group II, 1000 U/kg; and group III, 5000 U/kg. Growth, body weight, the weight of main organs, blood haematological and biochemical parameters were evaluated. Gene expression in the liver were analyzed using RNA-seq and qPCR techniques.RESULTS:
We observed a lower platelet count (p < 0,008) and a significantly greater (p < 0.02) number of WBCs in rats supplemented with 1000 U/kg than in rats from group III (5000 U/kg). Moreover, we noted a trend (p < 0.06) in total cholesterol concentration, suggesting a linear decrease with increasing doses of vitamin D. RNA-seq analysis did not reveal any differentially expressed genes with FDR < 0.05. However, GSEA revealed significant activation of a number of processes and pathways, including "metallothionein, and TspO/MBR family", and "negative regulation of tumor necrosis factor production". qPCR analysis revealed significant upregulation of the Mt1, Mt2 and Orm1 genes in animals receiving high doses of vitamin D (p < 0.025, p < 0.025, and p < 0009, respectively). Moreover, Srebp2 and Insig2 were significantly lower in both experimental groups than in the control group (p < 0.003 and p < 0.036, respectively).CONCLUSIONS:
Our results support the anti-inflammatory, anitioxidant and anticholesterologenic potential of vitamin D but suggest that high doses of vitamin D are needed to obtain significant results in this regard.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Vitamina D
/
Colecalciferol
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article