A novel vitrified cryopreservation approach with stem cell-laden hydrogel microcapsules.
Cryo Letters
; 45(2): 114-121, 2024.
Article
em En
| MEDLINE
| ID: mdl-38557990
ABSTRACT
BACKGROUND:
Stem cell-laden hydrogel microcapsules construction is important for a wide application in tissue engineering and cell-based medicine, such as building an ideal immune barrier. Challenges are emerging for effectively storing such microcapsules by cryopreservation, and a large proportion of research has been on the cryopreservation of single cells encapsulated into microcapsules without a core-shell structure.OBJECTIVE:
To achieve the effective cryopreservation of stem cell-laden hydrogel microcapsules with a core-shell structure. MATERIALS ANDMETHODS:
A novel core-shell alginate hydrogel encapsulation method was used to produce mesenchymal stem cell-laden microcapsules by microfluidic technique.RESULTS:
This microcapsule could inhibit ice formation to achieve vitreous cryopreservation with a low concentration (2 M) of penetrating cryoprotectants.CONCLUSION:
Cell laden hydrogel microcapsules may have the potential to be the basis of a new strategy of cell cryopreservation and applications. https//doi.org/10.54680/fr24210110212.
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Base de dados:
MEDLINE
Assunto principal:
Hidrogéis
/
Células-Tronco Mesenquimais
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article