Marinobacter azerbaijanicus sp. nov., a moderately halophilic bacterium from Urmia Lake, Iran.
Int J Syst Evol Microbiol
; 74(4)2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38568082
ABSTRACT
A novel moderately halophilic, Gram-stain-negative and facultatively anaerobic bacterium, designated as strain TBZ242T, was isolated from water of Urmia Lake in the Azerbaijan region of Iran. The cells were found to be rod-shaped and motile by a single polar flagellum, producing circular and yellowish colonies. The strain could grow in the presence of 0.5-10â% (w/v) NaCl (optimum, 2.5-5â%). The temperature and pH ranges for growth were 15-45â°C (optimum 30â°C) and pH 7.0-11.0 (optimum pH 8.0) on marine agar. The 16S rRNA gene sequence analysis revealed that strain TBZ242T belonged to the genus Marinobacter, showing the highest similarities to Marinobacter algicola DG893T (98.8â%), Marinobacter vulgaris F01T (98.8â%), Marinobacter salarius R9SW1T (98.5â%), Marinobacter panjinensis PJ-16T (98.4â%), Marinobacter orientalis W62T (98.0â%) and Marinobacter denitrificans JB2H27T (98.0â%). The 16S rRNA and core-genome phylogenetic trees showed that strain TBZ242T formed a distinct branch, closely related to a subclade accommodating M. vulgaris, M. orientalis, M. panjinensis, M. denitrificans, M. algicola, M. salarius and M. iranensis, within the genus Marinobacter. Average nucleotide identity and digital DNA-DNA hybridization values between strain TBZ242T and the type strains of the related species of Marinobacter were ≤85.0 and 28.6â%, respectively, confirming that strain TBZ242T represents a distinct species. The major cellular fatty acids of strain TBZ242T were C16ââ0 and C16ââ1 ω7c/C16ââ1 ω6c and the quinone was ubiquinone Q-9. The genomic DNA G+C content of strain TBZ242T is 57.2 mol%. Based on phenotypic, chemotaxonomic and genomic data, strain TBZ242T represents a novel species within the genus Marinobacter, for which the name Marinobacter azerbaijanicus sp. nov. is proposed. The type strain is TBZ242T (= CECT 30649T = IBRC-M 11466T). Genomic fragment recruitment analysis showed that this species prefers aquatic saline environments with intermediate salinities, being detected on metagenomic databases of Lake Meyghan (Iran) with 5 and 18â% salinity, respectively.
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Base de dados:
MEDLINE
Assunto principal:
Marinobacter
/
Ácidos Graxos
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article