Optimization of fermentation conditions and medium components for chrysomycin a production by Streptomyces sp. 891-B6.
BMC Microbiol
; 24(1): 120, 2024 Apr 06.
Article
em En
| MEDLINE
| ID: mdl-38582825
ABSTRACT
BACKGROUND:
Chrysomycin A (CA) is a promising antibiotic for treatment of Gram-positive bacterial infections and cancers. In order to enhance CA yield, optimization of fermentation conditions and medium components was carried out on strain Streptomyces sp. 891-B6, an UV-induced mutant with improved CA titer compared with its wide-type marine strain 891.RESULTS:
Using one-way experiment, the optimal fermentation conditions for CA production in 1-L shake flask were obtained as follows 12 days of fermentation time, 5 days of seed age, 5% of inoculum volume ratio, 200 mL of loading volume and 6.5 of initial pH. By response surface methodology, the optimal medium components determined as glucose (39.283 g/L), corn starch (20.662 g/L), soybean meal (15.480 g/L) and CaCO3 (2.000 g/L).CONCLUSION:
Validation tests showed that the maximum yield of CA reached 1601.9 ± 56.7 mg/L, which was a 60% increase compared to the initial yield (952.3 ± 53.2 mg/L). These results provided an important basis for scale-up production of CA by strain 891-B6.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Streptomyces
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article