Your browser doesn't support javascript.
loading
A chromosome-level genome assembly of the soybean pod borer: insights into larval transcriptional response to transgenic soybean expressing the pesticidal Cry1Ac protein.
Wang, Yangzhou; Yao, Yao; Zhang, Yunyue; Qian, Xueyan; Guo, Dongquan; Coates, Brad S.
Afiliação
  • Wang Y; Jilin Academy of Agricultural Sciences, Changchun, 130033, China.
  • Yao Y; Jilin Academy of Agricultural Sciences, Changchun, 130033, China.
  • Zhang Y; Jilin Academy of Agricultural Sciences, Changchun, 130033, China.
  • Qian X; Jilin Academy of Agricultural Sciences, Changchun, 130033, China.
  • Guo D; Jilin Academy of Agricultural Sciences, Changchun, 130033, China. xzgdq@126.com.
  • Coates BS; United States Department of Agriculture, Agricultural Research Service, Corn Insects & Crop Genetics Research Unit, 532 Science II, 2310 Pammel Dr., Ames, IA, 50011, USA. brad.coates@usda.gov.
BMC Genomics ; 25(1): 355, 2024 Apr 09.
Article em En | MEDLINE | ID: mdl-38594617
ABSTRACT

BACKGROUND:

Genetically modified (GM) crop plants with transgenic expression of Bacillus thuringiensis (Bt) pesticidal proteins are used to manage feeding damage by pest insects. The durability of this technology is threatened by the selection for resistance in pest populations. The molecular mechanism(s) involved in insect physiological response or evolution of resistance to Bt is not fully understood.

RESULTS:

To investigate the response of a susceptible target insect to Bt, the soybean pod borer, Leguminivora glycinivorella (Lepidoptera Tortricidae), was exposed to soybean, Glycine max, expressing Cry1Ac pesticidal protein or the non-transgenic parental cultivar. Assessment of larval changes in gene expression was facilitated by a third-generation sequenced and scaffolded chromosome-level assembly of the L. glycinivorella genome (657.4 Mb; 27 autosomes + Z chromosome), and subsequent structural annotation of 18,197 RefSeq gene models encoding 23,735 putative mRNA transcripts. Exposure of L. glycinivorella larvae to transgenic Cry1Ac G. max resulted in prediction of significant differential gene expression for 204 gene models (64 up- and 140 down-regulated) and differential splicing among isoforms for 10 genes compared to unexposed cohorts. Differentially expressed genes (DEGs) included putative peritrophic membrane constituents, orthologs of Bt receptor-encoding genes previously linked or associated with Bt resistance, and those involved in stress responses. Putative functional Gene Ontology (GO) annotations assigned to DEGs were significantly enriched for 36 categories at GO level 2, respectively. Most significantly enriched cellular component (CC), biological process (BP), and molecular function (MF) categories corresponded to vacuolar and microbody, transport and metabolic processes, and binding and reductase activities. The DEGs in enriched GO categories were biased for those that were down-regulated (≥ 0.783), with only MF categories GTPase and iron binding activities were bias for up-regulation genes.

CONCLUSIONS:

This study provides insights into pathways and processes involved larval response to Bt intoxication, which may inform future unbiased investigations into mechanisms of resistance that show no evidence of alteration in midgut receptors.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Praguicidas / Bacillus thuringiensis / Mariposas Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Praguicidas / Bacillus thuringiensis / Mariposas Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article