The polysaccharides from Balanophora polyandra enhanced neuronal autophagy to ameliorate brain function decline in natural aging mice through the PI3K/AKT/mTOR signaling pathway.
Neuroreport
; 35(8): 509-517, 2024 05 15.
Article
em En
| MEDLINE
| ID: mdl-38597274
ABSTRACT
The decline of aging brain neurons is the main cause of various neurodegenerative disease. This study aimed to examine the impact of Balanophora polyandra polysaccharides (BPP) against aging related neuronal deterioration. C57BL/6 mice were fed with regular feed for 27â
months to establish a natural aging mouse model. From 3â
months of age, mice in the drug-treated group were respectively fed with feed containing 0.05 or 0.18% BPP until 27â
months of age. The effects of BPP treatment on the pathological changes of neurons in mice brain were evaluated, as well as autophagy-related and signaling pathway proteins. BPP treatment had a notable positive impact on the pathological injury of cortical and hippocampal neurons, alleviated neuronal degeneration, and enhanced the staining of Nissl bodies in natural aging mice. Furthermore, BPP upregulated autophagy-related proteins LC3 II/I, Parkin, and PINK1 in the cortex and hippocampus of aging mice, and significantly decreased the expression of p62, PI3K, p-protein Kinase B (AKT), and p-mTOR. Immunofluorescence results showed a reduction in the brightness of LC3, which mainly coexpressed with NeuN in natural aging mice brain, and increased LC3-positive neurons were observed after BPP treatment. Collectively, BPP treatment enhanced neuronal autophagy to improve brain functional degradation through the PI3K/AKT/mTOR signaling in natural aging mice. These finding suggested that BPP has potential to mitigate or delay the neurodegeneration associated with aging and further investigation was needed to validate its efficacy in elderly populations.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Autofagia
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Encéfalo
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Envelhecimento
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Transdução de Sinais
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Fosfatidilinositol 3-Quinases
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Proteínas Proto-Oncogênicas c-akt
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Serina-Treonina Quinases TOR
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Camundongos Endogâmicos C57BL
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Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article