Single-cell senescence identification reveals senescence heterogeneity, trajectory, and modulators.
Cell Metab
; 36(5): 1126-1143.e5, 2024 May 07.
Article
em En
| MEDLINE
| ID: mdl-38604170
ABSTRACT
Cellular senescence underlies many aging-related pathologies, but its heterogeneity poses challenges for studying and targeting senescent cells. We present here a machine learning program senescent cell identification (SenCID), which accurately identifies senescent cells in both bulk and single-cell transcriptome. Trained on 602 samples from 52 senescence transcriptome datasets spanning 30 cell types, SenCID identifies six major senescence identities (SIDs). Different SIDs exhibit different senescence baselines, stemness, gene functions, and responses to senolytics. SenCID enables the reconstruction of senescent trajectories under normal aging, chronic diseases, and COVID-19. Additionally, when applied to single-cell Perturb-seq data, SenCID helps reveal a hierarchy of senescence modulators. Overall, SenCID is an essential tool for precise single-cell analysis of cellular senescence, enabling targeted interventions against senescent cells.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Senescência Celular
/
Análise de Célula Única
/
Transcriptoma
/
Aprendizado de Máquina
/
COVID-19
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article