Your browser doesn't support javascript.
loading
Deficiency of the BKCa potassium channel displayed significant implications for the physiology of the human bronchial epithelium.
Maliszewska-Olejniczak, Kamila; Pytlak, Karolina; Dabrowska, Adrianna; Zochowska, Monika; Hoser, Jakub; Lukasiak, Agnieszka; Zajac, Miroslaw; Kulawiak, Bogusz; Bednarczyk, Piotr.
Afiliação
  • Maliszewska-Olejniczak K; Department of Physics and Biophysics, Institute of Biology, Warsaw University of Life Sciences - SGGW, Warsaw, Poland.
  • Pytlak K; Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland.
  • Dabrowska A; Department of Physics and Biophysics, Institute of Biology, Warsaw University of Life Sciences - SGGW, Warsaw, Poland.
  • Zochowska M; Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland.
  • Hoser J; Department of Physics and Biophysics, Institute of Biology, Warsaw University of Life Sciences - SGGW, Warsaw, Poland.
  • Lukasiak A; Department of Physics and Biophysics, Institute of Biology, Warsaw University of Life Sciences - SGGW, Warsaw, Poland.
  • Zajac M; Department of Physics and Biophysics, Institute of Biology, Warsaw University of Life Sciences - SGGW, Warsaw, Poland.
  • Kulawiak B; Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland. Electronic address: b.kulawiak@nencki.edu.pl.
  • Bednarczyk P; Department of Physics and Biophysics, Institute of Biology, Warsaw University of Life Sciences - SGGW, Warsaw, Poland. Electronic address: piotr_bednarczyk@sggw.edu.pl.
Mitochondrion ; 76: 101880, 2024 May.
Article em En | MEDLINE | ID: mdl-38604459
ABSTRACT
Plasma membrane large-conductance calcium-activated potassium (BKCa) channels are important players in various physiological processes, including those mediated by epithelia. Like other cell types, human bronchial epithelial (HBE) cells also express BKCa in the inner mitochondrial membrane (mitoBKCa). The genetic relationships between these mitochondrial and plasma membrane channels and the precise role of mitoBKCa in epithelium physiology are still unclear. Here, we tested the hypothesis that the mitoBKCa channel is encoded by the same gene as the plasma membrane BKCa channel in HBE cells. We also examined the impact of channel loss on the basic function of HBE cells, which is to create a tight barrier. For this purpose, we used CRISPR/Cas9 technology in 16HBE14o- cells to disrupt the KCNMA1 gene, which encodes the α-subunit responsible for forming the pore of the plasma membrane BKCa channel. Electrophysiological experiments demonstrated that the disruption of the KCNMA1 gene resulted in the loss of BKCa-type channels in the plasma membrane and mitochondria. We have also shown that HBE ΔαBKCa cells exhibited a significant decrease in transepithelial electrical resistance which indicates a loss of tightness of the barrier created by these cells. We have also observed a decrease in mitochondrial respiration, which indicates a significant impairment of these organelles. In conclusion, our findings indicate that a single gene encodes both populations of the channel in HBE cells. Furthermore, this channel is critical for maintaining the proper function of epithelial cells as a cellular barrier.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Brônquios / Células Epiteliais / Subunidades alfa do Canal de Potássio Ativado por Cálcio de Condutância Alta Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Brônquios / Células Epiteliais / Subunidades alfa do Canal de Potássio Ativado por Cálcio de Condutância Alta Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article