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Direct detection of acetylcholinesterase by Fe(HCOO)2.6(OH)0.3. H2O nanosheets with oxidase-like activity on a smartphone platform.
Cao, Yongbin; Chen, Yuanyuan; Zhou, Yue; Chen, Xiaohua; Peng, Jian.
Afiliação
  • Cao Y; State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan, 430070, China; School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, China.
  • Chen Y; Department of Pharmacology, Medical College, Wuhan University of Science and Technology, Wuhan, 430022, China.
  • Zhou Y; State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan, 430070, China; School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, China.
  • Chen X; Department of Laboratory Medicine, General Hospital of Central Theater Command, Wuhan, 430070, China.
  • Peng J; State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan, 430070, China; School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070, China. Electronic address: jianpeng@whut.edu.cn.
Talanta ; 274: 126074, 2024 Jul 01.
Article em En | MEDLINE | ID: mdl-38608632
ABSTRACT
Monitoring acetylcholinesterase (AChE) is crucial in clinical diagnosis and drug screening. Traditional methods for detecting AChE usually require the addition of intermediates like acetylthiocholine, which complicates the detection process and introduces interference risks. Herein, we develop a direct colorimetric assay based on alkaline iron formate nanosheets (Fe(HCOO)2.6(OH)0.3·H2O NSs, Fef NSs) for the detection of AChE without any intermediates. The as-prepared Fef NSs exhibit oxidase-like activity, catalyzing the generation of O2·-, 1O2 and ·OH, which leads to a color change from colorless to blue when exposed to 3,3',5,5'-tetramethylbenzidine. AChE directly inhibits the oxidase-like activity of Fef NSs, resulting in a hindered color reaction, enabling the detection of AChE. The biosensor has a linear detection range of 0.1-30 mU/mL, with a minimum detection limit of 0.0083 mU/mL (S/N = 3), representing a 100-fold improvement in detection sensitivity over the traditional Ellman's method. Satisfactory results were obtained when analyzing real AChE samples. Attractively, a method for the quantitative detection of AChE by a smartphone is established based on the Fef NSs. This method enables instant acquisition of AChE concentrations, achieving real-time visualized detection.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Acetilcolinesterase / Técnicas Biossensoriais / Colorimetria / Nanoestruturas / Smartphone Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Acetilcolinesterase / Técnicas Biossensoriais / Colorimetria / Nanoestruturas / Smartphone Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article