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Deciphering the role of brainstem glycinergic neurons during startle and prepulse inhibition.
Huang, Wanyun; Cano, Jose C; Fénelon, Karine.
Afiliação
  • Huang W; Biology Department, University of Massachusetts Amherst, Life Science Laboratories, 240 Thatcher Road, Amherst, MA, 01002, USA.
  • Cano JC; Department of Biological Sciences, University of Texas at El Paso, 500 West University Avenue, El Paso, TX, 79912, USA.
  • Fénelon K; Biology Department, University of Massachusetts Amherst, Life Science Laboratories, 240 Thatcher Road, Amherst, MA, 01002, USA. Electronic address: kfenelon@umass.edu.
Brain Res ; 1836: 148938, 2024 Aug 01.
Article em En | MEDLINE | ID: mdl-38615924
ABSTRACT
Prepulse inhibition (PPI) of the auditory startle response, a key measure of sensorimotor gating, diminishes with age and is impaired in various neurological conditions. While PPI deficits are often associated with cognitive impairments, their reversal is routinely used in experimental systems for antipsychotic drug screening. Yet, the cellular and circuit-level mechanisms of PPI remain unclear, even under non-pathological conditions. We recently showed that brainstem neurons located in the caudal pontine reticular nucleus (PnC) expressing the glycine transporter type 2 (GlyT2±) receive inputs from the central nucleus of the amygdala (CeA) and contribute to PPI but via an uncharted pathway. Here, using tract-tracing, immunohistochemistry and in vitro optogenetic manipulations coupled to field electrophysiological recordings, we reveal the neuroanatomical distribution of GlyT2± PnC neurons and PnC-projecting CeA glutamatergic neurons and we provide mechanistic insights on how these glutamatergic inputs suppress auditory neurotransmission in PnC sections. Additionally, in vivo experiments using GlyT2-Cre mice confirm that optogenetic activation of GlyT2± PnC neurons enhances PPI and is sufficient to induce PPI in young mice, emphasizing their role. However, in older mice, PPI decline is not further influenced by inhibiting GlyT2± neurons. This study highlights the importance of GlyT2± PnC neurons in PPI and underscores their diminished activity in age-related PPI decline.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Reflexo de Sobressalto / Tronco Encefálico / Proteínas da Membrana Plasmática de Transporte de Glicina / Inibição Pré-Pulso / Glicina / Neurônios Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Reflexo de Sobressalto / Tronco Encefálico / Proteínas da Membrana Plasmática de Transporte de Glicina / Inibição Pré-Pulso / Glicina / Neurônios Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article