Direct Determination of the Structure of Single Biopolymer Molecules Using Nanopore Sequencing.
Biochemistry (Mosc)
; 89(Suppl 1): S234-S248, 2024 Jan.
Article
em En
| MEDLINE
| ID: mdl-38621753
ABSTRACT
This review highlights operational principles, features, and modern aspects of the development of third-generation sequencing technology of biopolymers focusing on the nucleic acids analysis, namely the nanopore sequencing system. Basics of the method and technical solutions used for its realization are considered, from the first works showing the possibility of creation of these systems to the easy-to-handle procedure developed by Oxford Nanopore Technologies company. Moreover, this review focuses on applications, which were developed and realized using equipment developed by the Oxford Nanopore Technologies, including assembly of whole genomes, methagenomics, direct analysis of the presence of modified bases.
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Base de dados:
MEDLINE
Assunto principal:
Nanoporos
/
Sequenciamento por Nanoporos
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article