A Mitochondria-Targeted Nitric Oxide Probe for Multimodality Imaging of Macrophage Immune Responses.
Anal Chem
; 96(17): 6666-6673, 2024 04 30.
Article
em En
| MEDLINE
| ID: mdl-38623755
ABSTRACT
Nitric oxide (NO) is a crucial signal molecule closely linked to the biological immune response, especially in macrophage polarization. When activated, macrophages enter a pro-inflammatory state and produce NO, a marker for the M1 phenotype. In contrast, the anti-inflammatory M2 phenotype does not produce NO. We developed a mitochondria-targeted two-photon iridium-based complex (Ir-ImNO) probe that can detect endogenous NO and monitor macrophages' different immune response states using various imaging techniques, such as one- and two-photon phosphorescence imaging and phosphorescence lifetime imaging. Ir-ImNO was used to monitor the immune activation of macrophages in mice. This technology aims to provide a clear and comprehensive visualization of macrophage immune responses.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Macrófagos
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Mitocôndrias
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Óxido Nítrico
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article