Genetic diversity in Leishmania infantum and Leishmania tropica isolates from human and canine hosts in northern Morocco.
Gene
; 921: 148484, 2024 Aug 30.
Article
em En
| MEDLINE
| ID: mdl-38642857
ABSTRACT
This study investigated nine provinces in northern Morocco and collected 275 skin scraping, 22 bone marrow aspirates, and 89 fine needle aspirations from suspected cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL) patients and potentially infected dogs. Molecular analysis using ITS1 RFLP PCR and RT-PCR revealed a higher prevalence of L. infantum (66.18 %; χ2 = 28.804; df = 1; P-value = 8.01e-08) than L. tropica in skin scraping, with L. infantum being the sole causative agent for both VL and canine leishmaniasis. L. infantum was predominantly found in most provinces, while L. tropica was relatively more dominant in Taza Province. Discriminant Analysis of Principal Components (DAPC) revealed distinct clustering between L. tropica and the other three species. However, no small subset of SNPs could clearly differentiate between Infantum_CL, Infantum_VL, and CanL, as they likely share a significant genetic background. The high rate of L. infantum could be attributed to the abundance of sand fly species transmitting VL. In Taza Province, Phlebotomus sergenti, responsible for anthroponotic CL, is the most abundant species. DNA sequencing demonstrated sequence heterogeneity in L. infantum (variants 1-9) and L. tropica (variants 1-7). Phylogenetic analysis showed a distinct separation between L. tropica and L. infantum strains, with an overlap among L. infantum strains isolated from cutaneous, visceral, and canine cases, and dogs serving as the central population for L. infantum.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Variação Genética
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Leishmania tropica
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Leishmania infantum
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Doenças do Cão
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Leishmaniose Visceral
Limite:
Animals
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Humans
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Male
País como assunto:
Africa
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article