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Evaluation puramatrix as a 3D microenvironment for neural differentiation of human breastmilk stem cells.
Goudarzi, Nasim; Shabani, Ronak; Moradi, Fatemeh; Ebrahimi, Marzieh; Katebi, Majid; Jafari, Amir; Mehdinejadiani, Shayesteh; Vahabzade, Gelareh; Soleimani, Mansoure.
Afiliação
  • Goudarzi N; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Anatomical Sciences, Cellular and Molecular Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran.
  • Shabani R; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Anatomy, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.
  • Moradi F; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Anatomy, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.
  • Ebrahimi M; Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. Electronic address: mebrahimi@royaninstitute.org.
  • Katebi M; Department of Anatomy, Faculty of Medical Science, Bandarabas, Hormozgan University of Medical Sciences, Hormozgan, Iran.
  • Jafari A; Laboratório de Neurofisiologia, Instituto de Biologia Roberto Alcantara Gomes, Centro Biomédico, Universidade do Estado do Rio de Janeiro. Electronic address: jafari.amir@posgraduacao.uerj.br.
  • Mehdinejadiani S; Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Vahabzade G; Department of Pharmacology, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.
  • Soleimani M; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Department of Anatomy, Faculty of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran. Electronic address: soleimani.m@iums.ac.ir.
Brain Res ; 1836: 148936, 2024 Aug 01.
Article em En | MEDLINE | ID: mdl-38649134
ABSTRACT
The extracellular matrix is recognized as an efficient and determining component in the growth, proliferation, and differentiation of cells due to its ability to perceive and respond to environmental signals. Applying three-dimensional scaffolds can create conditions similar to the extracellular matrix and provide an opportunity to investigate cell fate. In this study, we employed the PuraMatrix hydrogel scaffold as an advanced cell culture platform for the neural differentiation of stem cells derived from human breastmilk to design an opportune model for tissue engineering. Isolated stem cells from breastmilk were cultured and differentiated into neural-like cells on PuraMatrix peptide hydrogel and in the two-dimensional system. The compatibility of breastmilk-derived stem cells with PuraMatrix and cell viability was evaluated by scanning electron microscopy and MTT assay, respectively. Induction of differentiation was achieved by exposing cells to the neurogenic medium. After 21 days of the initial differentiation process, the expression levels of glial fibrillary acidic protein (GFAP), microtubule-associated protein (MAP2), ß-tubulin III, and neuronal nuclear antigen (NeuN) were analyzed using the immunostaining technique. The results illustrated a notable expression of MAP2, ß-tubulin-III, and NeuN in the three-dimensional cell culture in comparison to the two-dimensional system, indicating the beneficial effect of PuraMatrix scaffolds in the process of differentiating breastmilk-derived stem cells into neural-like cells. In view of the obtained results, the combination of breastmilk-derived stem cells and PuraMatrix hydrogel scaffold could be an advisable preference for neural tissue regeneration and cell therapy.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Leite Humano Limite: Female / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Leite Humano Limite: Female / Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article