New liquid chromatography tandem mass spectrometry reference data for estradiol show mini-puberty in both sexes and typical pre-pubertal and pubertal patterns.
Eur J Endocrinol
; 190(5): 401-408, 2024 May 02.
Article
em En
| MEDLINE
| ID: mdl-38652605
ABSTRACT
CONTEXT Reliable estradiol (E2) reference intervals (RIs) are crucial in pediatric endocrinology. OBJECTIVES:
This study aims to develop a sensitive ultra-performance liquid chromatographic tandem mass spectrometry (UPLC-MS/MS) method for E2 in serum, to establish graphically represented RI percentiles and annual RIs for both sexes, and to perform a systematic literature comparison.METHODS:
First, a UPLC-MS/MS method for E2 was developed. Second, graphically represented RI percentiles and annual RIs covering 0-18 years were computed (cohort of healthy children [1181 girls and 543 boys]). Subsequently, RIs were compared with published data by systematic searches.RESULTS:
Lower limit of quantification was 11â pmol/L, indicating high sensitivity. Estradiol first peaked during mini-puberty in both sexes (girls up to 192â pmol/L; boys up to 225â pmol/L). As could be expected, girls showed higher pubertal E2 (up to 638â pmol/L). However, boys' RIs (up to 259â pmol/L) overlapped considerably. We found 4 studies in the literature that also used LC-MS/MS to determine E2 and published RIs for the complete pediatric age range. Reference intervals varied considerably. Pre-pubertal and pubertal phases were present in all studies. Higher E2 during the time of mini-puberty in both sexes was documented in 3 studies including ours.CONCLUSIONS:
Variability of RIs for E2 between studies illustrates the importance of laboratory-specific RIs despite using a LC-MS/MS reference method. In boys, the striking E2 peak during mini-puberty as well as high pubertal E2 without phenotypic estrogenization in regular male puberty indicates that the role of E2 in children and, especially in boys, requires better functional understanding.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Puberdade
/
Estradiol
/
Espectrometria de Massas em Tandem
Limite:
Adolescent
/
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
/
Newborn
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article