Gamma irradiation in modulating arsenic bioremediation potential of Pseudomonas sp. AK1 and AK9.
Int J Radiat Biol
; 100(6): 934-939, 2024.
Article
em En
| MEDLINE
| ID: mdl-38657135
ABSTRACT
PURPOSE:
Present study deals with the role of gamma irradiation in modulating arsenic bioremediation of Pseudomonas sp. AK1 and AK9 strains. MATERIALS ANDMETHODS:
The bacterial strains AK1 and AK9 of Pseudomonas sp. were irradiated at different doses (5 Gy, 10 Gy, 15 Gy and 20 Gy) of gamma irradiation. The effect of γ-irradiation on the growth and arsenic modulating ability of AK1 and AK9 strains was determined in the presence and absence of arsenic along with non-irradiated strains. Further, a comparative study of non-irradiated and irradiated strains by protein profiling in absence and presence of arsenic was carried out to confirm of the increased expression ofarsenite oxidase.RESULTS:
Both strains were able to transform AsIII to AsV. Both strains AK1 and AK9 decrease the arsenic concentration by 626.68 ppb (13.36%) and 686.40 ppb (14.71%) after an incubation period of 96 h in presence of arsenic. Gamma irradiated AK9 strains showed doubled growth in presence of arsenic as compared to non-irradiated strains at 10 Gy treatment whereas no changes in growth was observed in irradiated AK1 strains. Gamma irradiated AK9 strain decrease 378.65 ppb (7.27%) more arsenic concentration from natural water sample supplemented with AsIII than non-irradiated AK9 strain. Further, in the protein profile, increased expression of arsenite oxidase (â¼85 kDa) was observed in irradiated AK9 strains in presence of arsenic.CONCLUSIONS:
Overall, the results suggested that the gamma irradiated AK9 strain having potential for arsenic accumulation and increased arsenite tolerance may play a great role in the bioremediation of the arsenite at arsenic contaminated sites.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Arsênio
/
Pseudomonas
/
Biodegradação Ambiental
/
Raios gama
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article