An efficient lipid droplet-targeted fluorescent probe for detection of intracellular viscosity.
Luminescence
; 39(4): e4749, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38658767
ABSTRACT
Lipid droplet, an intracellular lipid reservoir, is vital for energy metabolism and signal transmission in cells. The viscosity directly affects the metabolism of lipid droplets, and the abnormal viscosity is associated with the occurrence and development of various diseases. Therefore, it is indispensable to develop techniques that can detect viscosity changes in intracellular lipid droplets. Based on twisted intramolecular charge transfer (TICT) mechanism, a novel small-molecule lipid droplet-targeted viscosity fluorescence probe PPF-1 was designed. The probe was easy to synthesize, it had a large Stokes shift, stable optical properties, and low bio-toxicity. Compared to being in methanol solution, the fluorescence intensity of PPF-1 in glycerol solution was increased 26.7-fold, and PPF-1 showed excellent ability to target lipid droplets. Thus, the probe PPF-1 could provide an effective means of detecting viscosity changes of lipid droplets and was of great value for physiological diagnosis of related diseases, pathological analysis, and medical research.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Gotículas Lipídicas
/
Corantes Fluorescentes
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article