Evaluation of different genomic regions of rotavirus B and rotavirus C for development of real-time RTâPCR assays.
Virol J
; 21(1): 94, 2024 04 24.
Article
em En
| MEDLINE
| ID: mdl-38659036
ABSTRACT
BACKGROUND:
The causative agents of diarrhea, rotavirus B (RVB) and rotavirus C (RVC) are common in adults and patients of all age groups, respectively. Due to the Rotavirus A (RVA) vaccination program, a significant decrease in the number of gastroenteritis cases has been observed globally. The replacement of RVA infections with RVB, RVC, or other related serogroups is suspected due to the possibility of reducing natural selective constraints due to RVA infections. The data available on RVB and RVC incidence are scant due to the lack of cheap and rapid commercial diagnostic assays and the focus on RVA infections. The present study aimed to develop real-time RTâPCR assays using the data from all genomic RNA segments of human RVB and RVC strains available in the Gene Bank.RESULTS:
Among the 11 gene segments, NSP3 and NSP5 of RVB and the VP6 gene of RVC were found to be suitable for real-time RTâPCR (qRTâPCR) assays. Fecal specimens collected from diarrheal patients were tested simultaneously for the presence of RVB (n = 192) and RVC (n = 188) using the respective conventional RTâPCR and newly developed qRTâPCR assays. All RVB- and RVC-positive specimens were reactive in their respective qRTâPCR assays and had Ct values ranging between 23.69 and 41.97 and 11.49 and 36.05, respectively. All known positive and negative specimens for other viral agents were nonreactive, and comparative analysis showed 100% concordance with conventional RTâPCR assays.CONCLUSIONS:
The suitability of the NSP5 gene of RVB and the VP6 gene of RVC was verified via qRTâPCR assays, which showed 100% sensitivity and specificity. The rapid qRTâPCR assays developed will be useful diagnostic tools, especially during diarrheal outbreaks for testing non-RVA rotaviral agents and reducing the unnecessary use of antibiotics.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Infecções por Rotavirus
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Rotavirus
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Diarreia
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Fezes
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Reação em Cadeia da Polimerase em Tempo Real
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article