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Glycogen phase separation drives macromolecular rearrangement and asymmetric division in E. coli.
Thappeta, Yashna; Cañas-Duarte, Silvia J; Kallem, Till; Fragasso, Alessio; Xiang, Yingjie; Gray, William; Lee, Cheyenne; Cegelski, Lynette; Jacobs-Wagner, Christine.
Afiliação
  • Thappeta Y; Sarafan Chemistry, Engineering, and Medicine for Human Health Institute, Stanford University, Stanford, CA, USA.
  • Cañas-Duarte SJ; Department of Biology, Stanford University, Stanford, CA, USA.
  • Kallem T; Sarafan Chemistry, Engineering, and Medicine for Human Health Institute, Stanford University, Stanford, CA, USA.
  • Fragasso A; Howard Hughes Medical Institute, Stanford University, Stanford, USA.
  • Xiang Y; Department of Chemistry, Stanford University, Stanford, CA, USA.
  • Gray W; Sarafan Chemistry, Engineering, and Medicine for Human Health Institute, Stanford University, Stanford, CA, USA.
  • Lee C; Department of Biology, Stanford University, Stanford, CA, USA.
  • Cegelski L; Mechanical Engineering and Materials Science, Yale University, New Haven, CT.
  • Jacobs-Wagner C; Mechanical Engineering and Materials Science, Yale University, New Haven, CT.
bioRxiv ; 2024 Apr 20.
Article em En | MEDLINE | ID: mdl-38659787
ABSTRACT
Bacteria often experience nutrient limitation in nature and the laboratory. While exponential and stationary growth phases are well characterized in the model bacterium Escherichia coli, little is known about what transpires inside individual cells during the transition between these two phases. Through quantitative cell imaging, we found that the position of nucleoids and cell division sites becomes increasingly asymmetric during transition phase. These asymmetries were coupled with spatial reorganization of proteins, ribosomes, and RNAs to nucleoid-centric localizations. Results from live-cell imaging experiments, complemented with genetic and 13C whole-cell nuclear magnetic resonance spectroscopy studies, show that preferential accumulation of the storage polymer glycogen at the old cell pole leads to the observed rearrangements and asymmetric divisions. In vitro experiments suggest that these phenotypes are likely due to the propensity of glycogen to phase separate in crowded environments, as glycogen condensates exclude fluorescent proteins under physiological crowding conditions. Glycogen-associated differences in cell sizes between strains and future daughter cells suggest that glycogen phase separation allows cells to store large glucose reserves without counting them as cytoplasmic space.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article