DNA Isolation Long-Read Genomic Sequencing in Ctenophores.
Methods Mol Biol
; 2757: 185-200, 2024.
Article
em En
| MEDLINE
| ID: mdl-38668967
ABSTRACT
Long-read sequencing has proven the necessity for high-quality genomic assemblies of reference species, including enigmatic ctenophores. Obtaining high-molecular-weight genomic DNA is pivotal to this process and has proven highly problematic for many species. Here, we discuss different methodologies for gDNA isolation and present a protocol for isolating gDNA for several members of the phylum Ctenophora. Specifically, we describe a Pacific Biosciences library construction method used in conjunction with gDNA isolation methods that have proven successful in obtaining high-quality genomic assemblies in ctenophores.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
DNA
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Análise de Sequência de DNA
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Genômica
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Ctenóforos
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article