The Drosophila Hippo pathway transcription factor Scalloped and its co-factors alter each other's chromatin binding dynamics and transcription in vivo.
Dev Cell
; 59(13): 1640-1654.e5, 2024 Jul 08.
Article
em En
| MEDLINE
| ID: mdl-38670104
ABSTRACT
The Hippo pathway is an important regulator of organ growth and cell fate. The major mechanism by which Hippo is known to control transcription is by dictating the nucleo-cytoplasmic shuttling rate of Yorkie, a transcription co-activator, which promotes transcription with the DNA binding protein Scalloped. The nuclear biophysical behavior of Yorkie and Scalloped, and whether this is regulated by the Hippo pathway, remains unexplored. Using multiple live-imaging modalities on Drosophila tissues, we found that Scalloped interacts with DNA on a broad range of timescales, and enrichment of Scalloped at sites of active transcription is mediated by longer DNA dwell times. Further, Yorkie increased Scalloped's DNA dwell time, whereas the repressors Nervous fingers 1 (Nerfin-1) and Tondu-domain-containing growth inhibitor (Tgi) decreased it. Therefore, the Hippo pathway influences transcription not only by controlling nuclear abundance of Yorkie but also by modifying the DNA binding kinetics of the transcription factor Scalloped.
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Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
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Cromatina
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Transdução de Sinais
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Transativadores
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Proteínas Serina-Treonina Quinases
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Proteínas de Drosophila
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Drosophila melanogaster
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Proteínas de Sinalização YAP
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article