Your browser doesn't support javascript.
loading
Enzymatic fluorescent supramolecular hydrogel with aggregation-induced emission characteristics for sensing alkaline phosphatase.
Deng, Yun; Fu, Cheng; Xu, Aifei; He, Rongxiang; Lu, Wangting; Liu, Min.
Afiliação
  • Deng Y; Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute for Interdisciplinary Research, College of Photoelectric Materials and Technology, Jianghan University, Wuhan 430056, Hubei, China. Electronic address: ustcdy@jhun.edu.cn.
  • Fu C; Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute for Interdisciplinary Research, College of Photoelectric Materials and Technology, Jianghan University, Wuhan 430056, Hubei, China.
  • Xu A; School of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, China.
  • He R; Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute for Interdisciplinary Research, College of Photoelectric Materials and Technology, Jianghan University, Wuhan 430056, Hubei, China.
  • Lu W; Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute for Interdisciplinary Research, College of Photoelectric Materials and Technology, Jianghan University, Wuhan 430056, Hubei, China.
  • Liu M; Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute for Interdisciplinary Research, College of Photoelectric Materials and Technology, Jianghan University, Wuhan 430056, Hubei, China. Electronic address: mliu2018@jhun.edu.cn.
Spectrochim Acta A Mol Biomol Spectrosc ; 316: 124334, 2024 Aug 05.
Article em En | MEDLINE | ID: mdl-38678837
ABSTRACT
Alkaline phosphatase is an important biomarker for medical diagnosis. An enzymatic fluorescence supramolecular hydrogel with AIE properties was developed and used for sensing alkaline phosphatase in vitro and in living cells. In the presence of ALP, K(TPE)EFYp was partially converted to the hydrogelator K(TPE)EFY and self-assembled into nanofibers to form Hydrogel. With the sol-gel transition and the AIE effect, the fluorescence emission was turned on. The linear concentration range of ALP activity in vitro quantified by this method was determined as 0-3 U/L with aLODat 0.02 U/L. In addition, cell imaging and serum experiment showed that K(TPE)EFYp could also be used to detect ALP activity in living cells and biological samples.
Assuntos
Palavras-chave
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Fluorescência / Hidrogéis / Fosfatase Alcalina Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Fluorescência / Hidrogéis / Fosfatase Alcalina Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article