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Sphingomonas bacteria could serve as an early bioindicator for the development of chlorantraniliprole resistance in Spodoptera frugiperda.
Gu, Meng; Lv, Shenglan; Hu, Mengfan; Yang, Ziyi; Xiao, Yuying; Wang, Xuegui; Liang, Pei; Zhang, Lei.
Afiliação
  • Gu M; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
  • Lv S; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
  • Hu M; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
  • Yang Z; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
  • Xiao Y; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
  • Wang X; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China; College of Agriculture, Sichuan Agricultural University, Chengdu 611130, China.
  • Liang P; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
  • Zhang L; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China. Electronic address: zhanglei86@sina.com.
Pestic Biochem Physiol ; 201: 105891, 2024 May.
Article em En | MEDLINE | ID: mdl-38685253
ABSTRACT
The fall armyworm (Spodoptera frugiperda) was found to have invaded China in December 2018, and in just one year, crops in 26 provinces were heavily affected. Currently, the most effective method for emergency control of fulminant pests is to use of chemical pesticides. Recently, most fall armyworm populations in China were begining to exhibite low level resistance to chlorantraniliprole. At present, it is not possible to sensitively reflect the low level resistance of S. frugiperda by detecting target mutation and detoxification enzyme activity. In this study we found that 12 successive generations of screening with chlorantraniliprole caused S. frugiperda to develop low level resistance to this insecticide, and this phenotype was not attribute to genetic mutations in S. frugiperda, but rather to a marked increase in the relative amount of the symbiotic bacteria Sphingomonas. Using FISH and qPCR assays, we determined the amount of Sphingomonas in the gut of S. frugiperda and found Sphingomonas accumulation to be highest in the 3rd-instar larvae. Additionally, Sphingomonas was observed to provide a protective effect to against chlorantraniliprole stress to S. frugiperda. With the increase of the resistance to chlorantraniliprole, the abundance of bacteria also increased, we propose Sphingomonas monitoring could be adapted into an early warning index for the development of chlorantraniliprole resistance in S. frugiperda populations, such that timely measures can be taken to delay or prevent the widespread propagation of resistance to this highly useful agricultural chemical in S. frugiperda field populations.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Spodoptera / Sphingomonas / Ortoaminobenzoatos / Inseticidas / Larva Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Spodoptera / Sphingomonas / Ortoaminobenzoatos / Inseticidas / Larva Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article