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Glyphosate presence in human sperm: First report and positive correlation with oxidative stress in an infertile French population.
Vasseur, Claudine; Serra, Loïse; El Balkhi, Souleiman; Lefort, Gaëlle; Ramé, Christelle; Froment, Pascal; Dupont, Joëlle.
Afiliação
  • Vasseur C; Centre de fertilité, Pôle Santé Léonard de Vinci, Chambray-lès-Tours, France. Electronic address: claudine.vasseur@pslv.fr.
  • Serra L; CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly F-37380, France.
  • El Balkhi S; Service de Pharmacologie, Toxicologie et Pharmacovigilance, Limoges, CHU F-87042, France.
  • Lefort G; CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly F-37380, France.
  • Ramé C; CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly F-37380, France.
  • Froment P; CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly F-37380, France.
  • Dupont J; CNRS, IFCE, INRAE, Université de Tours, PRC, Nouzilly F-37380, France. Electronic address: joelle.dupont@inrae.fr.
Ecotoxicol Environ Saf ; 278: 116410, 2024 Jun 15.
Article em En | MEDLINE | ID: mdl-38696871
ABSTRACT
Environmental exposure to endocrine disruptors, such as pesticides, could contribute to a decline of human fertility. Glyphosate (GLY) is the main component of Glyphosate Based Herbicides (GBHs), which are the most commonly herbicides used in the world. Various animal model studies demonstrated its reprotoxicity. In Europe, GLY authorization in agriculture has been extended until 2034. Meanwhile the toxicity of GLY in humans is still in debate. The aims of our study were firstly to analyse the concentration of GLY and its main metabolite, amino-methyl-phosphonic acid (AMPA) by LC/MS-MS in the seminal and blood plasma in an infertile French men population (n=128). We secondly determined Total Antioxidant Status (TAS) and Total Oxidant Status (TOS) using commercial colorimetric kits and some oxidative stress biomarkers including malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) by ELISA assays. We next analysed potential correlations between GLY and oxidative stress biomarkers concentration and sperm parameters (sperm concentration, progressive speed, anormal forms). Here, we detected for the first time GLY in the human seminal plasma in significant proportions and we showed that its concentration was four times higher than those observed in blood plasma. At the opposite, AMPA was undetectable. We also observed a strong positive correlation between plasma blood GLY concentrations and plasma seminal GLY and 8-OHdG concentrations, the latter reflecting DNA impact. In addition, TOS, Oxidative Stress Index (OSI) (TOS/TAS), MDA blood and seminal plasma concentrations were significantly higher in men with glyphosate in blood and seminal plasma, respectively. Taken together, our results suggest a negative impact of GLY on the human reproductive health and possibly on his progeny. A precaution principle should be applied at the time of the actual discussion of GLY and GBHs formulants uses in Europe by the authorities.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espermatozoides / Estresse Oxidativo / Glifosato / Glicina / Herbicidas / Infertilidade Masculina Limite: Adult / Humans / Male / Middle aged País como assunto: Europa Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espermatozoides / Estresse Oxidativo / Glifosato / Glicina / Herbicidas / Infertilidade Masculina Limite: Adult / Humans / Male / Middle aged País como assunto: Europa Idioma: En Ano de publicação: 2024 Tipo de documento: Article