Temporal dynamics and genomic programming of plasma cell fates.
Nat Immunol
; 25(6): 1097-1109, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38698087
ABSTRACT
Affinity-matured plasma cells (PCs) of varying lifespans are generated through a germinal center (GC) response. The developmental dynamics and genomic programs of antigen-specific PC precursors remain to be elucidated. Here, using a model antigen in mice, we demonstrate biphasic generation of PC precursors, with those generating long-lived bone marrow PCs preferentially produced in the late phase of GC response. Clonal tracing using single-cell RNA sequencing and B cell antigen receptor sequencing in spleen and bone marrow compartments, coupled with adoptive transfer experiments, reveals a new PC transition state that gives rise to functionally competent PC precursors. The latter undergo clonal expansion, dependent on inducible expression of TIGIT. We propose a model for the proliferation and programming of precursors of long-lived PCs, based on extended antigen encounters in the GC.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasmócitos
/
Diferenciação Celular
/
Centro Germinativo
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article