Sensitive and effective method with 96-well plate for determination of levamlodipine in human plasma using LC-MS/MS.
Anal Biochem
; 691: 115556, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-38705226
ABSTRACT
we developed an effective protein precipitation method for determination of levamlodipine in human plasma using LC-MS/MS. Sample extraction was carried out by using liquid-liquid extraction in 96-well plate format. (S)-Amlodipine-d4 was used as internal standard (IS). The chromatographic separation was achieved using Philomen Chiral MX (2) column (3 µm, 2.1 × 100 mm). Mobile phase A was comprised of Acetonitrile (ACN), Mono ethanol amine (MEA) and Iso-Propyl alcohol (IPA) (1000110, v/v/v), Mobile phase B was IPA-ACN (21, v/v). The flow rate was 0.4 mL/min. The total run time of each sample was 4.0 min with gradient elution. LC-MS/MS spectra were generated in positive ion mode, and multiple reaction monitoring (MRM) was used to detect the following transitions m/z 409.20 â 238.15 for levamlodipine and 415.25 â 240.20 for (S)-Amlodipine-d4 (the IS). The method was linear from 50 to 10000 pg/mLï¼R2ï¼0.9988489ï¼,and the lower limit of quantification (LLOQ) was 50 pg/mL. This method was applied to a bioequivalence study of levamlodipine.
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MEDLINE
Assunto principal:
Niacina
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Humans
Idioma:
En
Ano de publicação:
2024
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Article