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Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway.
Liang, Yu; Zhao, Bishi; Shen, Yan; Peng, Miao; Qiao, Liying; Liu, Jianhua; Pan, Yangyang; Yang, Kaijie; Liu, Wenzhong.
Afiliação
  • Liang Y; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
  • Zhao B; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
  • Shen Y; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
  • Peng M; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
  • Qiao L; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
  • Liu J; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
  • Pan Y; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
  • Yang K; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
  • Liu W; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
Int J Mol Sci ; 25(9)2024 Apr 23.
Article em En | MEDLINE | ID: mdl-38731807
ABSTRACT
Fat tissue-a vital energy storage organ-is intricately regulated by various factors, including circular RNA, which plays a significant role in modulating fat development and lipid metabolism. Therefore, this study aims to clarify the regulatory mechanism of sheep adipocyte proliferation and differentiation by investigating the involvement of circTIAM1, miR-485-3p, and its target gene PLCB1. Through previous sequencing data, circTIAM1 was identified in sheep adipocytes, with its circularization mechanism elucidated, confirming its cytoplasmic localization. Experimental evidence from RNase R treatment and transcription inhibitors highlighted that circTIAM1 is more stable than linear RNA. Additionally, circTIAM1 promoted sheep adipocyte proliferation and differentiation. Furthermore, bioinformatic analysis demonstrated a robust interaction between miR-485-3p and circTIAM1. Further experiments revealed that miR-485-3p inhibits fat cell proliferation and differentiation by inhibiting PLCB1, with circTIAM1 alleviating the inhibitory effect via competitive binding. In summary, our findings elucidate the mechanism through which circTIAM1 regulates Guangling Large-Tailed sheep adipocyte proliferation and differentiation via the miR-485-3p-PLCB1 pathway, offering a novel perspective for further exploring fat metabolism regulation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Adipócitos / MicroRNAs / Fosfolipase C beta / RNA Circular Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Adipócitos / MicroRNAs / Fosfolipase C beta / RNA Circular Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article