The effect of miR-23b-3p on regulating GH by targeting POU1F1 in Yanbian yellow cattle.
Anim Biotechnol
; 35(1): 2346808, 2024 Nov.
Article
em En
| MEDLINE
| ID: mdl-38739483
ABSTRACT
This study aimed to evaluate the effect of miR-23b-3p on growth hormone (GH) in pituitary cells of Yanbian yellow cattle. The mRNA and protein levels of GH and miR-23b-3p target genes were measured by real time fluorescence quantitative PCR (qPCR) and Western blot, respectively. The target relationship of miR-23b-3p was validated by double luciferase reporter gene system. The results showed that GH mRNA and protein levels in pituitary cells of Yanbian yellow cattle were significantly lower in the miR-23b-3p-mi group than in the NC group (P<0.01), while GH mRNA and protein levels were higher in the miR-23b-3p-in group than in the iNC group (P<0.05). The result of bioinformatics analysis and double luciferase reporter gene system validation proved that miR-23b-3p targeted 3'UTR of pituitary specific transcription factor 1 (POU1F1). POU1F1 mRNA and protein levels were lower miR-23b-3p-mi group than in the NC group (P<0.01), while POU1F1 mRNA and protein levels were higher in the miR-23b-3p-in group than in the iNC group (P<0.01). These results demonstrated that miR-23b-3p could regulate GH expression in pituitary cells by regulating POU1F1 gene.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Hormônio do Crescimento
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MicroRNAs
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Fator de Transcrição Pit-1
Limite:
Animals
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article