Delayed gramicidin delivery through an intra-pipette capillary facilitates perforated patch recordings.
Neurosci Res
; 2024 May 11.
Article
em En
| MEDLINE
| ID: mdl-38740268
ABSTRACT
The gramicidin-perforated patch-clamp technique is indispensable for recording neuronal activities without changing the intracellular Cl- concentration. Conventionally, gramicidin contained in the pipette fluid is delivered to the cell membrane by passive diffusion. Gramicidin deposited on the pipette orifice sometimes hampers giga-seal formation, and perforation progresses only slowly. These problems may be circumvented by delivering a high concentration of gramicidin from an intra-pipette capillary after a giga-seal is formed. We herein describe the detailed protocol of this improved method. This protocol would greatly facilitate the investigation of Cl- gradient-dependent neuronal activities.
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MEDLINE
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En
Ano de publicação:
2024
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Article