Protocol to generate scaffold-free, multicomponent 3D melanoma spheroid models for preclinical drug testing.
STAR Protoc
; 5(2): 103058, 2024 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-38748881
ABSTRACT
Three-dimensional (3D) models play an increasingly important role in preclinical drug testing as they faithfully mimic interactions between cancer cells and the tumor microenvironment (TME). Here, we present a protocol for generating scaffold-free 3D multicomponent human melanoma spheroids. We describe steps for characterizing models using live-cell imaging and histology, followed by drug testing and assessment of cell death through various techniques such as imaging, luminescence-based assays, and flow cytometry. Finally, we demonstrate the models' adaptability for co-cultures with immune cells.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Esferoides Celulares
/
Melanoma
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article